Rabbit Anti-DNA Ligase III antibody

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DNA Ligase 3; D11Wsu78e; DNA ligase; DNA ligase 3; DNA ligase III; DNLI3_HUMAN; LIG 3; LIG2; LIG3; Ligase II, DNA, ATP dependent; Ligase III DNA ATP dependent; MGC78176; OTTMUSP00000000875; Polydeoxyribonucleotide synthase [ATP] 3; Polydeoxyribonucleotide

Cat:

SL10754R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human DNA Ligase III:701-800/1009

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-DNA Ligase III Polyclonal Antibody, Unconjugated(SL10754R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-DNA Ligase III Polyclonal Antibody, Unconjugated(SL10754R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.

Function:
Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.

Subcellular Location:
Nucleus.

Tissue Specificity:
Testis, thymus, prostate and heart.

Similarity:
Belongs to the ATP-dependent DNA ligase family.
Contains 1 BRCT domain.
Contains 1 PARP-type zinc finger.

SWISS:
P49916

Gene ID:
796

Database links:

Entrez Gene: 514719 Cow

Entrez Gene: 796 Human

Entrez Gene: 16882 Mouse

Entrez Gene: 303369 Rat

Omim: 600940 Human

SwissProt: P49916 Human

SwissProt: P97386 Mouse

Unigene: 100299 Human

Unigene: 277136 Mouse

Unigene: 230056 Rat

Picture

Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-DNA Ligase III Polyclonal Antibody, Unconjugated(SL10754R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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