ZFAND2B is a 257 amino acid protein containing two AN1-type zinc fingers and two UIM (ubiquitin-interacting motif) repeats. Conserved in animals and plants, the AN1-type zinc finger domain is often found in proteins that contain a ubiquitin-like domain, which suggests a role in the ubiquitination pathway. There are two isoforms of ZFAND2B that are produced as a result of alternative splicing events.
Subcellular Location:
Endoplasmic reticulum.
Similarity:
Contains 2 AN1-type zinc fingers.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
SWISS:
Q8WV99
Gene ID:
130617
Database links:
Entrez Gene: 130617 Human
Entrez Gene: 68818 Mouse
Entrez Gene: 363253 Rat
Omim: 613474 Human
SwissProt: Q8WV99 Human
SwissProt: Q91X58 Mouse
SwissProt: Q4KLG9 Rat
Unigene: 534540 Human
Unigene: 32646 Mouse
Unigene: 32162 Rat
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Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFAND2B) Polyclonal Antibody, Unconjugated (SL18519R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFAND2B) Polyclonal Antibody, Unconjugated (SL18519R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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