Rabbit Anti-ZCCHC4 antibody

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4930449I23Rik; HSPC052; MGC21108; OTTMUSP00000027189; zcchc4; ZCHC4_HUMAN; Zinc finger CCHC domain containing protein 4; Zinc finger CCHC domain-containing protein 4; Zinc finger, CCHC domain containing 4.

Cat:

SL18553R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human ZCCHC4:301-400/513

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Jurkat(Human) Cell Lysate at 30 ugRaji(Human) Cell Lysate at 30 ugPrimary: Anti-ZCCHC4 (SL18553R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 59 kDObserved band size: 59 kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZCCHC4) Polyclonal Antibody, Unconjugated (SL18553R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ZCCHC4 is a 513 amino acid protein that contains one CCHSLCtype zinc finger and one DHHSLCtype zinc finger and is thought to function as a methyltransferase. The gene encoding ZCCHC4 maps to human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease.

Function:
May be a methyltransferase.

Similarity:
Contains 1 CCHSLCtype zinc finger.
Contains 1 DHHSLCtype zinc finger.

SWISS:
Q9H5U6

Gene ID:
29063

Database links:

Entrez Gene: 511397 Cow

Entrez Gene: 29063 Human

Omim: 611792 Human

SwissProt: E1BGQ2 Cow

SwissProt: Q9H5U6 Human

Unigene: 278945 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZCCHC4) Polyclonal Antibody, Unconjugated (SL18553R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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