Rabbit Anti-ZCCHC6 antibody

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KIAA1711; MGC38585; PAP associated domain containing 6; PAPD6; Zinc finger CCHC domain containing 6.

Cat:

SL18555R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Pig,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human ZCCHC6:111-210/1495

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-ZCCHC6 (SL18555R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 171 kDObserved band size: 171 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZCCHC6) Polyclonal Antibody, Unconjugated (SL18555R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ZCCHC6 is a 1,495 amino acid uridylyltransferase that mediates RNA uridylation. A member of the DNA polymerase type-B-like family, ZCCHC6 contains three CCHSLCtype zinc fingers and two PAP-associated domains, and exists as six alternatively spliced isoforms. The gene encoding ZCCHC6 maps to human chromosome 9q21.33 and mouse chromosome 13 B2.

Function:
Uridylyltransferase that mediates the terminal uridylation of some specific RNAs. Not involved in uridylation of precursor let-7 (pre-let-7) miRNA. Does not play a role in replication-dependent histone mRNA degradation.

Similarity:
Belongs to the DNA polymerase type-B-like family.
Contains 3 CCHSLCtype zinc fingers.
Contains 2 PAP-associated domains.

SWISS:
Q5VYS8

Gene ID:
79670

Database links:

Entrez Gene: 79670 Human

SwissProt: Q5VYS8 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZCCHC6) Polyclonal Antibody, Unconjugated (SL18555R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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