Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities. [provided by RefSeq, Jul 2008]
Function:
Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (By similarity). May have weak lysophospholipase activity.
Subcellular Location:
Lysosome. Secreted.
Tissue Specificity:
Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Post-translational modifications:
N-glycosylated.
Similarity:
Belongs to the AB hydrolase superfamily. Lipase family.
SWISS:
Q8NCC3
Gene ID:
23659
Database links:
Entrez Gene: 282271 Cow
Entrez Gene: 403403 Dog
Entrez Gene: 23659 Human
Entrez Gene: 192654 Mouse
Entrez Gene: 361401 Rat
Omim: 609362 Human
SwissProt: Q8NCC3 Human
SwissProt: Q8VEB4 Mouse
SwissProt: Q675A5 Rat
Unigene: 632199 Human
Unigene: 284770 Mouse
Unigene: 93631 Rat
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Sample:
293T(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Primary: Anti-LYPLA3 (SL18579R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZC3HAV1L) Polyclonal Antibody, Unconjugated (SL18531R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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