Rabbit Anti-MANSC1 antibody

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9130403P13Rik; FLJ10298; LOH12CR3; Loss of heterozygosity 12 chromosomal region 3 protein; MANS1_HUMAN; MANSC domain containing 1; MANSC domain-containing protein 1; MANSC1.

Cat:

SL18656R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human MANSC1:27-130/431<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Large intestine(Mouse) Cell Lysate at 40 ugPrimary: Anti-MANSC1 (SL18656R ) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 44 kDObserved band size: 49 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MANSC1) Polyclonal Antibody, Unconjugated (SL18656R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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MANSC1 is a 414 amino acid single-pass membrane protein. Expressed throughout the body, MANSC1 contains one MANSC domain and is encoded by a gene that is located on chromosome 12. Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12, including hypochondrogenesis, achondrogenesis and Kniest dysplasia. Chromosome 12 is also home to a homeobox gene cluster which encodes crucial transcription factors for morphogenesis, and the natural killer complex gene cluster encoding SLCtype lectin proteins which mediate the NK cell response to MHC I interaction.

Subcellular Location:
Membrane.

Tissue Specificity:
Widely expressed.

Similarity:
Contains 1 MANSC domain.

SWISS:
Q9H8J5

Gene ID:
54682

Database links:

Entrez Gene: 54682 Human

Entrez Gene: 67729 Mouse

Entrez Gene: 690606 Rat

SwissProt: Q9H8J5 Human

SwissProt: Q9CR33 Mouse

Unigene: 591145 Human

Unigene: 57648 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MANSC1) Polyclonal Antibody, Unconjugated (SL18656R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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