Rabbit Anti-MAP1D antibody | Sunlong Medical

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AMP1D_HUMAN; CDS of metAP 3 within PCR fragment; M ethionine aminopeptidase 1D, mitochondrial precursor; MAP 1D; MAP1D; MetAP 1D; Metap1l; Methionine aminopeptidase 1D; Methionine aminopeptidase 1D, mitochondrial; Methionyl aminopeptidase type 1D

Cat:

SL18659R

Species Reactivity：

Human,(predicted: Mouse,Dog,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human MAP1D:251-335/335

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: SW96(Human) Cell Lysate at 30 ugPrimary: Anti-MAP1D (SL18659R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 35 kDObserved band size: 35 kDParaformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1D) Polyclonal Antibody, Unconjugated (SL18659R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]

Function:
Removes the amino-terminal methionine from nascent proteins (By similarity). May play a role in colon tumorigenesis.

Subcellular Location:
Mitochondrion.

Tissue Specificity:
Overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level).

Similarity:
Belongs to the peptidase M24A family.

SWISS:
Q6UB28

Gene ID:
254042

Database links:

Entrez Gene: 254042 Human

Entrez Gene: 66559 Mouse

Entrez Gene: 311748 Rat

Omim: 610267 Human

SwissProt: Q6UB28 Human

SwissProt: Q9CPW9 Mouse

Unigene: 298250 Human

Unigene: 152796 Mouse

Unigene: 4826 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP1D) Polyclonal Antibody, Unconjugated (SL18659R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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