Rabbit Anti-MAP3K13/LZK antibody

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EC 2.7.11.25; Leucine zipper bearing kinase; Leucine zipper bearing kinase; Leucine zipper-bearing kinase; LZK; M3K13_HUMAN; MAP3K13; MEKK13; MGC133196; Mitogen activated protein kinase kinase kinase 13; Mitogen-activated protein kinase kinase k

Cat:

SL18663R

Species Reactivity：

Human,Mouse,(predicted: Cow,Orangutan)

Immunogen：

KLH conjugated synthetic peptide derived from human MAP3K13/LZK:801-900/966

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: K562 Cell (Human) Lysate at 40 ugPrimary: Anti-MAP3K13/LZK (SL18663R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 108 kDObserved band size: 108 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP3K13) Polyclonal Antibody, Unconjugated (SL18663R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008]

Function:
Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.

Subcellular Location:
Cytoplasm. Membrane.

Tissue Specificity:
Expressed in the adult brain, liver, placenta and pancreas, with expression strongest in the pancreas.

Post-translational modifications:
Autophosphorylated on serine and threonine residues.

Similarity:
Belongs to the protein kinase superfamily.
STE Ser/Thr protein kinase family.
MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.

SWISS:
O43283

Gene ID:
9175

Database links:

Entrez Gene: 9175 Human

Entrez Gene: 71751 Mouse

Omim: 604915 Human

SwissProt: O43283 Human

SwissProt: Q1HKZ5 Mouse

Unigene: 656069 Human

Unigene: 420837 Mouse

Unigene: 438136 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP3K13) Polyclonal Antibody, Unconjugated (SL18663R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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