This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008]
Function:
Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Isoform 1 is testis- and sperm-specific.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the NASP family.
Contains 3 TPR repeats.
SWISS:
P49321
Gene ID:
4678
Database links:
Entrez Gene: 4678 Human
Entrez Gene: 512427 Cow
Entrez Gene: 50927 Mouse
Entrez Gene: 100009592 Rabbit
Entrez Gene: 298441 Rat
Omim: 603185 Human
SwissProt: Q2T9P4 Cow
SwissProt: P49321 Human
SwissProt: Q99MD9 Mouse
SwissProt: P27123 Rabbit
SwissProt: Q66HD3 Rat
Unigene: 319334 Human
Unigene: 257181 Mouse
Unigene: 48344 Rat
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Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NASP) Polyclonal Antibody, Unconjugated (SL19026R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-NASP Polyclonal Antibody, Unconjugated(SL19026R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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