Home > Product > Antibody > Rabbit Anti-DARC antibody
CCBP1; CD234; CD234 antigen; DARC; Dfy; Duffy antigen/chemokine receptor; Duffy blood group; DUFFY_MOUSE; FY antibody Fy glycoprotein; Glycoprotein D; GPD; GpFy; Human Duffy blood group antigen (Fya-b+) mRNA complete cds; Plasmodium vivax receptor; WBCQ1;
Cat:
SL10711R
Species Reactivity:
Human,Rat,
Immunogen:
KLH conjugated synthetic peptide derived from human DARC:61-150/336<Extracellular>
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-DARC Polyclonal Antibody, Unconjugated(SL10711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-DARC Polyclonal Antibody, Unconjugated(SL10711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Unit:
Price: $
Product PDFs
Datasheet:


Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.

Function:
Non-specific receptor for many chemokines.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in liver and brain.

Similarity:
Belongs to the G-protein coupled receptor Duffy family.

SWISS:
Q16570

Gene ID:
2532

Database links:

Entrez Gene: 2532 Human

Entrez Gene: 13349 Mouse

Entrez Gene: 681489 Rat

Omim: 110700 Human

SwissProt: Q16570 Human

SwissProt: Q9QUI6 Mouse

Unigene: 153381 Human

Unigene: 6393 Mouse



Picture

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-DARC Polyclonal Antibody, Unconjugated(SL10711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-DARC Polyclonal Antibody, Unconjugated(SL10711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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