Rabbit Anti-PRKCBP1 antibody

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ZMYND8; CTCL tumor antigen se14 3; Cutaneous T cell lymphoma associated antigen se14 3; KIAA1125; MGC31836; predicted protein of HQ2893; PRKCBP 1; PKCB1_HUMAN; PRO2893; Protein kinase C binding protein 1; Rack7; Zinc finger MYND domain containing

Cat:

SL19178R

Species Reactivity：

Human,Mouse,(predicted: Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human PRKCBP1:1-100/1186

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: A549(Human) Cell Lysate at 40 ugPrimary: Anti-PRKCBP1 (SL19178R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 119 kDObserved band size: 119 kD Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRKCBP1) Polyclonal Antibody, Unconjugated (SL19178R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a receptor for activated SLCkinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Subunit:
Interacts in vitro with PRKCB1.

Subcellular Location:
Nuclear

Tissue Specificity:
Expressed in all tissues examined with highest expression in brain, lung, pancreas, and placenta. Expressed in cutaneous T-cell lymphomas (CTCL).

Similarity:
Contains 1 bromo domain.

SWISS:
Q9ULU4

Gene ID:
23613

Database links:

Entrez Gene: 23613 Human

SwissProt: Q9ULU4 Human

Unigene: 44648 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRKCBP1) Polyclonal Antibody, Unconjugated (SL19178R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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