Rabbit Anti-Aquaporin 5 antibody

Home > Product > Antibody > Rabbit Anti-Aquaporin 5 antibody

Aquaporin 5; AQP5; AQP 5; AQP-5; AQP5_HUMAN; Aquaporin-5.

Cat:

SL10927R

Species Reactivity：

Human,Mouse,(predicted: Rat,Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human Aquaporin 5:151-250/265<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Eye (Rat) Lysate at 40 ugPrimary: Anti-Aquaporin 5 (SL10927R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 29 kDTissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Aquaporin Polyclonal Antibody, Unconjugated(SL10927R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008]

Function:
Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.

SWISS:
P55064

Gene ID:
362

Database links:

Entrez Gene: 362 Human

Entrez Gene: 11830 Mouse

Entrez Gene: 25241 Rat

Omim: 600442 Human

SwissProt: P55064 Human

SwissProt: Q9WTY4 Mouse

SwissProt: P47864 Rat

Unigene: 59623 Human

Unigene: 45580 Mouse

Unigene: 10066 Rat

Picture

Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Aquaporin Polyclonal Antibody, Unconjugated(SL10927R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall