The NPL4 gene encodes the 608-amino acid, endoplasmic reticulum and nuclear membrane protein Npl4 that forms a complex with p97/Cdc48p and Ufd1p. This complex plays a role in IP3 receptor processing by recognizing ubiquitinated IP3 receptors in the endoplasmic reticulum and delivering them to the proteasome for degradation. The Npl4 protein contains 8 potential N-myristoylation sites, 5 potential N-glycosylation sites, several phosphorylation sites, and a SLCterminal zinc finger motif. This protein is 96% homologous to the rat Npl4 protein, 44% homologous to the Caenorhabditis elegans Npl4 protein, and 34% homologous to the Saccharomyces cerevisiae Npl4 protein. Mutations in the NPL4 gene cause defects in nuclear envelope morphology, nuclear protein import, and nuclear poly(A) RNA export.
Function:
The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.
Subcellular Location:
Cytoplasm; cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear.
Tissue Specificity:
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
Similarity:
Belongs to the NPL4 family.
Contains 1 RanBP2-type zinc finger.
SWISS:
Q8TAT6
Gene ID:
55666
Database links:
Entrez Gene: 55666 Human
Entrez Gene: 217365 Mouse
Entrez Gene: 140639 Rat
Omim: 606590 Human
SwissProt: Q8TAT6 Human
SwissProt: P60670 Mouse
SwissProt: Q9ES54 Rat
Unigene: 464333 Human
Unigene: 309520 Mouse
Unigene: 144645 Rat
Unigene: 217306 Rat
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPL4) Polyclonal Antibody, Unconjugated (SL19334R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPL4) Polyclonal Antibody, Unconjugated (SL19334R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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