Rabbit Anti-NGFB antibody | Sunlong Medical

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NGF; Beta nerve growth factor; NGF-beta; Beta nerve growth factor precursor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor beta; Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGF B; NGFB; NID67; NGF beta; NGF-B; NGF_

Cat:

SL10806R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Sheep,)

Immunogen：

Recombinded human NGF beta full length protein

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Skin (Mouse) Lysate at 40 ug Lane 3: Thyroid gland (Mouse) Lysate at 40 ug Primary: Anti-NGFB (SL10806R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32/13 kD Observed band size: 35/13 kDSample: Lane 1: Mouse Brain Lysates Lane 2: Mouse Cerebellum Lysates Lane 3: Human U-87 MG cell Lysates Lane 4: Human SH-SY5Y cell Lysates Lane 5: Human U-2 OS cell Lysates Primary: Anti-NGFB (SL10806R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 13/32kDaObserved band size: 13/32kDaSample: Brain (Rat) Lysate at 40 ugPrimary: Anti-NGFB (SL10806R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 13 kDObserved band size: 15 kDTissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-NGFB Polyclonal Antibody, Unconjugated(SL10806R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingU87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (SL10806R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

Function:
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.

Subunit:
Homodimer.

Subcellular Location:
Secreted.

DISEASE:
Defects in NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) [MIM:608654]. The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self-mutilation. The autonomic involvement is variable.

Similarity:
Belongs to the NGF-beta family.

SWISS:
P0228

Gene ID:
963

Database links:

Entrez Gene: 963 Human

Entrez Gene: 3649 Mouse

Entrez Gene: 310738 Rat

Omim: 162030 Human

SwissProt: P0228 Human

SwissProt: P01139 Mouse

SwissProt: P25427 Rat

Unigene: 2561 Human

Unigene: 1259 Mouse

Unigene: 22168 Rat

Picture

Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Human U-87 MG cell Lysates
Lane 4: Human SH-SY5Y cell Lysates
Lane 5: Human U-2 OS cell Lysates
Primary: Anti-NGFB (SL10806R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/32kDa
Observed band size: 13/32kDa

Sample:
Brain (Rat) Lysate at 40 ug
Primary: Anti-NGFB (SL10806R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13 kD
Observed band size: 15 kD

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-NGFB Polyclonal Antibody, Unconjugated(SL10806R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (SL10806R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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