Rabbit Anti-Neural retinal specific leucine zipper/NRL antibody

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D14S46E; Neural retina-specific leucine zipper protein; Neural retinal specific leucine zipper; NRL; NRL MAF; NRL_HUMAN; RP27.

Cat:

SL19351R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Neural retinal specific leucine zipper/NRL:151-237/237

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Raji (human)cell Lysate at 40 ugPrimary: Anti-NRL(SL19351R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26 kDObserved band size: 30 kDTissue/cell: Rat eye tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-NRL Polyclonal Antibody, Unconjugated(SL19351R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a basic motif-leucine zipper transcription factor of the Maf subfamily. The encoded protein is conserved among vertebrates and is a critical intrinsic regulator of photoceptor development and function. Mutations in this gene have been associated with retinitis pigmentosa and retinal degenerative diseases. [provided by RefSeq, Jul 2008]

Function:
Transcription factor which regulates the expression of several rod-specific genes, in cluding RHO and PDE6B.

Subcellular Location:
Nucleus.

Tissue Specificity:
Neural retina.

DISEASE:
Defects in NRL are the cause of retinitis pigmentosa type 27 (RP27) [MIM:162080]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP27 inheritance is autosomal dominant.

Similarity:
Belongs to the bZIP family.
Contains 1 bZIP domain.

SWISS:
P54845

Gene ID:
4901

Database links:

Entrez Gene: 4901 Human

Entrez Gene: 18185 Mouse

Omim: 162080 Human

SwissProt: P54845 Human

SwissProt: P54846 Mouse

Unigene: 652297 Human

Unigene: 20422 Mouse

Picture

Tissue/cell: Rat eye tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-NRL Polyclonal Antibody, Unconjugated(SL19351R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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