Activator of SUMO1;
AOS 1;
AOS1;
FLJ3091;
HSPC 140;
HSPC140;
SAE 1;
Sae1;
SAE1_HUMAN;
Sentrin/SUMO activating protein AOS 1;
Sentrin/SUMO activating protein AOS1;
SUA 1;
SUA1;
SUMO 1 activating enzyme E1 N subunit;
SUMO 1 activating enzyme s
Cat:
SL19364R
Species Reactivity：
Mouse,(predicted: Human,Rat,)
Immunogen：
KLH conjugated synthetic peptide derived from human SAE1,Isoform 2:1-100/346
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: brain (mouse) Lysate at 40 ugPrimary: Anti-SAE1(SL19364R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 29 kDObserved band size: 29 kDSample: Large intestine (Mouse) Lysate at 40 ugPrimary: Anti- SAE1 (SL19364R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 30 kD
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
Subunit: Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I.
Subcellular Location: Nucleus.
Tissue Specificity: Expression level increases during S phase and drops in G2 phase (at protein level).
Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity: Belongs to the ubiquitin-activating E1 family.
Sample: brain (mouse) Lysate at 40 ug
Primary: Anti-SAE1(SL19364R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti- SAE1 (SL19364R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 30 kD
