Rabbit Anti-NUDCD1 antibody

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4921532K09Rik; AA407246; AW260430; AW556235; Chronic myelogenous leukemia tumor antigen 66; CML66; CML66 L; FLJ14991; NudC domain containing 1; NudC domain-containing protein 1; NUDC1_HUMAN; nudcd1; OVA66; RGD1310624; Tumor antigen CML66.

Cat:

SL19506R

Species Reactivity：

Mouse,(predicted: Human,Rat,Dog,Cow,Horse,Rabbit,Zebrafish,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human NUDCD1:431-530/583

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Thymus (Mouse) Lysate at 40 ugPrimary: Anti-NUDCD1 (SL19506R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 67 kDObserved band size: 63 kDParaformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUDCD1) Polyclonal Antibody, Unconjugated (SL19506R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The NUDCD1 gene encodes the 583 amino acid CML66 protein which demonstrates expression in a variety of solid tumor cell lines and leukemias. Expression of CML66 in normal tissues is restricted to testis and heart. The CML66 antibody is found in sera of 18-38% of patients with lung cancer, melanoma or prostate cancer. These findings indicate that CML66 may be immunogenic in a wide variety of malignancies and may be a target for antigen-specific immunotherapy. A short isoform of CML66, designated CML66-S, is a product of alternative splicing. CML66-S has the same SLCterminus as full-lenghth CML66 (also designated CML66-L), but has a unique N-terminus. CML66-S shows predominant expression in testis, and is also expressed in very low levels in tumor cells, while CML66 is expressed in tumor cells and testis.

Function:
Isoform 1 is specifically expressed in leukemias and a variety of solid tumor cell lines and is also detected in testis and heart. Isoform 2 is predominantly expressed in testis and weakly expressed in tumor cells.

Similarity:
Contains 1 CS domain.

SWISS:
Q96RS6

Gene ID:
84955

Database links:

Entrez Gene: 528425 Cow

Entrez Gene: 475073 Dog

Entrez Gene: 84955 Human

Entrez Gene: 67429 Mouse

Entrez Gene: 362906 Rat

Omim: 606109 Human

SwissProt: Q96RS6 Human

SwissProt: Q6PIP5 Mouse

Unigene: 380291 Human

Unigene: 292021 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUDCD1) Polyclonal Antibody, Unconjugated (SL19506R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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