Rabbit Anti-RAI14 antibody | Sunlong Medical

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Ankycorbin; Ankyrin repeat and coiled coil structure containing protein; Ankyrin repeat and coiled-coil structure-containing protein; DKFZp564G013; KIAA1334; NORPEG; Novel retinal pigment epithelial cell protein; RAI13; RAI14; RAI14_HUMAN; Retin

Cat:

SL19728R

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human RAI14:2-100/980

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T Cell (Human) Lysate at 30 ugPrimary: Anti-RAI14 (SL19728R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 110kDObserved band size: 120kDParaformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAI14) Polyclonal Antibody, Unconjugated (SL19728R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

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Picture

Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAI14) Polyclonal Antibody, Unconjugated (SL19728R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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