Rabbit Anti-RBM22 antibody | Sunlong Medical

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Cwc2; FLJ10290; fSAP47; Functional spliceosome associated protein 47; Pre mRNA splicing factor RBM22; Pre-mRNA-splicing factor RBM22; RBM 22; rbm22; RBM22_HUMAN; RNA binding motif protein 22; RNA-binding motif protein 22; ZC3H16; Zinc finger C

Cat:

SL19767R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human RBM22:101-300/420

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T(Human) Cell Lysate at 30 ugPrimary: Anti-RBM22 (SL19767R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 47 kDObserved band size: 47 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM22) Polyclonal Antibody, Unconjugated (SL19767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes an RNA binding protein. The encoded protein may play a role in cell division and may be involved in pre-mRNA splicing. Related pseudogenes exist on chromosomes 6, 7, 9, 13, 16, 18, and X. [provided by RefSeq, Mar 2009]

Function:
Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses.

Subcellular Location:
Nucleus. Cytoplasm. Mainly located in the nucleus. Translocated from the nucleus to the cytoplasm after heat shock cell treatment. May be shuttling between the nucleus and the cytosol.

Similarity:
Belongs to the SLT11 family.
Contains 1 C3H1-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
Q9NW64

Gene ID:
55696

Database links:

Entrez Gene: 512330 Cow

Entrez Gene: 489184 Dog

Entrez Gene: 55696 Human

Entrez Gene: 66810 Mouse

Entrez Gene: 100345063 Rabbit

Entrez Gene: 307410 Rat

Omim: 612430 Human

SwissProt: Q3B7L8 Cow

SwissProt: Q9NW64 Human

SwissProt: Q8SHS3 Mouse

SwissProt: Q4V7D7 Rat

Unigene: 713564 Human

Unigene: 275106 Mouse

Unigene: 105130 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM22) Polyclonal Antibody, Unconjugated (SL19767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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