Rabbit Anti-p66 beta/GATAD2B antibody

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C430014D17Rik; FLJ37346; GATA zinc finger domain containing 2B; GATA zinc finger domain containing protein 2B; GATA zinc finger domain-containing protein 2B; GATAD2B; KIAA1150; MGC138257; MGC138285; mKIAA1150; p66/p68; P66B_HUMAN; P66beta; RG

Cat:

SL21073R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human p66 beta:381-480/593

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: NIH/3T3(Mouse) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugPrimary: Anti-p66 beta (SL21073R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 66 kDObserved band size: 66 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p66 beta/GATAD2B) Polyclonal Antibody, Unconjugated (SL21073R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a zinc finger protein transcriptional repressor. The encoded protein is part of the methyl-CpG-binding protein-1 complex, which represses gene expression by deacetylating methylated nucleosomes. Mutations in this gene are linked to intellectual disability and dysmorphic features associated with mental retardation. [provided by RefSeq, Jun 2016]

Function:
Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.

Subcellular Location:
Nucleus. Nuclear, in discrete foci.

Tissue Specificity:
Ubiquitous.

Similarity:
Contains 1 GATA-type zinc finger.

SWISS:
Q8WXI9

Gene ID:
57459

Database links:

Entrez Gene: 57459 Human

Entrez Gene: 229542 Mouse

Entrez Gene: 310614 Rat

SwissProt: Q8WXI9 Human

SwissProt: Q8VHR5 Mouse

Unigene: 4779 Human

Unigene: 596854 Human

Unigene: 270999 Mouse

Unigene: 133230 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p66 beta/GATAD2B) Polyclonal Antibody, Unconjugated (SL21073R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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