The protein encoded by this gene is a scaffold protein and an intermediate signaling protein in the innate immune response pathway. The encoded protein helps transmit the immune response signal from Toll-like receptors to IRAK1/TRAF6 complexes. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Function:
Scaffold protein which probably links Toll-like receptors (TLRs) to basic cellular processes via its interaction with the complex containing IRAK kinases and TRAF6. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation. Probably involved in innate immune response.
Tissue Specificity:
Highly expressed in brain, heart and testis, and at lower level in kidney, liver, lung, placenta, small intestine, spleen and stomach. Isoform 1 is not expressed in lung.
Similarity:
Belongs to the pellino family.
SWISS:
Q8N2H9
Gene ID:
246330
Database links:
Entrez Gene: 246330 Human
Entrez Gene: 48518 Mouse
Entrez Gene: 309157 Rat
Omim: 609827 Human
SwissProt: Q8N2H9 Human
SwissProt: Q8BXR6 Mouse
Unigene: 656875 Human
Unigene: 277580 Mouse
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-PELI3 (SL12613R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PELI3) Polyclonal Antibody, Unconjugated (SL12613R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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