Processed zona pellucida sperm-binding protein 4;
ZBP;
Zona pellucida B protein;
Zona pellucida glycoprotein 4;
Zona pellucida protein B;
Zona pellucida sperm binding protein 4;
Zp-4;
ZP1;
ZP4;
ZP4_HUMAN;
ZPB.
Cat:
SL4470R
Species Reactivity：
Mouse,(predicted: Human,Rat,Horse,)
Immunogen：
KLH conjugated synthetic peptide derived from human ZP4:301-400/540<Extracellular>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Uterus(Mouse)Lysate at 40 ugPrimary: Anti-Zonapellucida glycoprotein 4(SL4470R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 57kDObserved band size: 57kDSample: Ovary (Mouse)Lysate at 40 ugPrimary: Anti-Zonapellucida glycoprotein 4(SL4470R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 57kDObserved band size: 57kD
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a SLCterminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008]
Function: The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.
Subcellular Location: Secreted > extracellular space > extracellular matrix and Cell membrane.
Tissue Specificity: Oocytes.
Post-translational modifications: Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
Similarity: Belongs to the ZP domain family. ZPB subfamily.
Contains 1 P-type (trefoil) domain.
Contains 1 ZP domain.
