Rabbit Anti-ALPPL2 antibody

Home > Product > Antibody > Rabbit Anti-ALPPL2 antibody

Akp5; Alkaline phosphatase; Alkaline phosphatase Nagao isozyme; Alkaline phosphatase placental like; ALP 1; ALP-1; ALP1; ALPG; ALPPL 2; ALPPL; C77216; D1Ertd816e; GCAP; Germ cell alkaline phosphatase; Nagao isozyme; Placental alkaline phosphatase-like; Pl

Cat:

SL20188R

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human ALPPL2:301-400/532

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ALPPL2 Polyclonal Antibody, Unconjugated(SL20188R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃). Primary Antibody (green line):Rabbit Anti-ALPPL2 antibody (SL20188R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008]

Subunit:
Homodimer.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity:
Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.

Similarity:
Belongs to the alkaline phosphatase family.

SWISS:
P10696

Gene ID:
251

Database links:

Entrez Gene: 251 Human

Omim: 171810 Human

SwissProt: P10696 Human

Unigene: 333509 Human

Picture

Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line):Rabbit Anti-ALPPL2 antibody (SL20188R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

Product Feedback Wall