Home > Product > Antibody > Rabbit Anti-OLFML1 antibody
MVAL564; Olfactomedin like 1; Olfactomedin like protein 1; OLFML 1; OLFML1; UNQ564.
Cat:
SL21082R
Species Reactivity:
Mouse,(predicted: Human,Rat,Dog,Horse,Rabbit,Sheep,)
Immunogen:
KLH conjugated synthetic peptide derived from human OLFML1:321-402/402
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Liver (Mouse) Lysate at 40 ugLung (Mouse) Lysate at 40 ugEye (Mouse) Lysate at 40 ugHippocampus (Mouse) Lysate at 40 ugPrimary: Anti- OLFML1 (SL21082R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 46 kDObserved band size: 48 kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OLFML1) Polyclonal Antibody, Unconjugated (SL21082R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Unit:
Price: $
Product PDFs
Datasheet:


OLFML1 (Olfactomedin-like protein 1) contains 1 olfactomedin-like domain. The specific function of this protein remains unknown.

Subcellular Location:
Secreted.

SWISS:
Q6UWY5

Gene ID:
283298

Database links:

Entrez Gene: 283298 Human

Entrez Gene: 244198 Mouse

Entrez Gene: 361621 Rat

SwissProt: Q6UWY5 Human

SwissProt: Q66H86 Rat



Picture

Sample:
Liver (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Eye (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- OLFML1 (SL21082R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OLFML1) Polyclonal Antibody, Unconjugated (SL21082R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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