Home > Product > Antibody > Rabbit Anti-PUS7L antibody
DKFZp781B2386; FLJ34711; FLJ40012; Pseudouridylate synthase 7 homolog; Pseudouridylate synthase 7 homolog (S. cerevisiae)-like; Pseudouridylate synthase 7 homolog-like protein; PUS7L.
Cat:
SL21112R
Species Reactivity:
Mouse,(predicted: Human,Rat,Dog,Pig,Cow,Horse,Sheep,)
Immunogen:
KLH conjugated synthetic peptide derived from human PUS7L:201-300/701
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PUS7L) Polyclonal Antibody, Unconjugated (SL21112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PUS7L) Polyclonal Antibody, Unconjugated (SL21112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Unit:
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Product PDFs
Datasheet:


DKFZP434G1415 also called PUS7L, belongs to the pseudouridine synthase truD family and contains 1 TRUD domain. The most abundant modification seen in structured RNAs (transfer, ribosomal, and splicing RNAs) is the isomerization of uridine (U) to pseudouridine (5-ribosyluracil). Pseudouridine is made by a set of enzymes called pseudouridine synthase, which select specific U residues in a polynucleotide chain for isomerization to pseudouridine.

SWISS:
Q9H0K6

Gene ID:
83448

Database links:

Entrez Gene: 83448 Human

SwissProt: Q9H0K6 Human

Unigene: 445814 Human



Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PUS7L) Polyclonal Antibody, Unconjugated (SL21112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PUS7L) Polyclonal Antibody, Unconjugated (SL21112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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