Rabbit Anti-RAP1GAP2 antibody

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DKFZp686O238; GARNL4; GTPase activating RANGAP domain like 4; GTPase activating Rap/RanGAP domain like 4; GTPase activating Rap/RanGAP domain like protein 4; KIAA1039; RAP1 GTPase activating protein 2; RAP1GA3.

Cat:

SL21155R

Species Reactivity：

Mouse,Rat,(predicted: Human,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human RAP1GAP2:651-730/730

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugPrimary: Anti- RAP1GAP2 (SL21155R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 80 kDObserved band size: 76 kDParaformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAP1GAP2) Polyclonal Antibody, Unconjugated (SL21155R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a GTPase-activating protein that activates the small guanine-nucleotide-binding protein Rap1 in platelets. The protein interacts with synaptotagmin-like protein 1 and Rab27 and regulates secretion of dense granules from platelets at sites of endothelial damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Tissue Specificity:
Cytoplasmic; perinuclear region.

Similarity:
Contains 1 RanBD1 domain.

SWISS:
Q684P5

Gene ID:
23108

Database links:

Entrez Gene: 23108 Human

Entrez Gene: 380711 Mouse

SwissProt: Q684P5 Human

SwissProt: Q5SVL6 Mouse

Unigene: 499659 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAP1GAP2) Polyclonal Antibody, Unconjugated (SL21155R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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