Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008]
Function: Involved in pre-mRNA splicing.
Subunit: Interacts directly with PRPF18, PPIH and PRPF3. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. This complex interacts with the U4/U5/U6 tri-snRNP complex.
Subcellular Location: Nucleus speckle. Note=Colocalizes with spliceosomal snRNPs.
Sample:
Raji (Human) Cell Lysate at 40 ug
HepG2 (Human) Cell Lysate at 40 ug
Hela (Human) Cell Lysate at 40 ug
Primary: Anti-PRPF4(SL20098R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD
Sample:
bone (mouse) Lysate at 40 ug
lymph node (mouse) Lysate at 40 ug
Primary: Anti-PRPF4(SL200098R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
