Rabbit Anti-DEF6/IBP antibody

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DEF-6; Def6; DEFI6_HUMAN; Differentially expressed in FDCP 6 homolog; Differentially expressed in FDCP 6 homologue mouse; IBP; IRF4-binding protein; Swap70 like adapter of T cells.

Cat:

SL20306R

Species Reactivity：

Rat,(predicted: Human,Mouse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human DEF6/IBP:51-150/631

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (rat thymus tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DEF6) Polyclonal Antibody, Unconjugated (SL20306R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DEF6) Polyclonal Antibody, Unconjugated (SL20306R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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DEF6 is a 631 amino acid protein that is widely expressed in T and B cells of the immune system. Localized to the nucleus, cytoplasm and cell membrane, DEF6 is a phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) that functions to activate Rho GTPases such as Rac 1, Cdc42 and Rho A. Via its ability to activate various Rho GTPases, DEF6 can regulate cell shape, movement and polarity and, through its interaction with T helper cells, DEF6 can also help mediate the immune response. DEF6 contains one PH (pleckstrin homology) domain which is required for binding to phosphatidylinositol 3,4,5-trisphosphate and subsequent recruitment to the plasma membrane.

Function:
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP-70 signaling.

Subunit:
Interacts with ZAP70 (By similarity). Interacts with IRF4, activated RAC1 and F-actin. Both the phosphorylated and non-phosphorylated forms bind phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3).

Subcellular Location:
Cytoplasm. Cell membrane. Nucleus. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate.

Tissue Specificity:
Broadly expressed in the immune system and can be detected in T and B-cells.

Post-translational modifications:
Tyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.

Similarity:
Contains 1 PH domain.

SWISS:
Q9H4E7

Gene ID:
50619

Database links:

Entrez Gene: 50619 Human

Entrez Gene: 23853 Mouse

Entrez Gene: 309642 Rat

Omim: 610094 Human

SwissProt: Q9H4E7 Human

SwissProt: Q8C2K1 Mouse

Unigene: 15476 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DEF6) Polyclonal Antibody, Unconjugated (SL20306R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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