Rabbit Anti-QPRT/QAPRTase antibody

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NADC_HUMAN; Nicotinate nucleotide pyrophosphorylase (carboxylating); Nicotinate-nucleotide pyrophosphorylase [carboxylating]; QAPRTase; QPRT; QPRTase; Quinolinate phosphoribosyltransferase [decarboxylating]; Quinolinate phosphoribosyltransferase.

Cat:

SL20316R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Cow,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human QPRT/QAPRTase :21-120/297

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (mouse) Lysate at 40 ugPrimary: Anti- QPRT'QAPRTase (SL20316R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 31kDObserved band size: 31kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Quinolinate phosphoribosyltransferase; QPRT/QAPRTase) Polyclonal Antibody, Unconjugated (SL20316R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Quinolinate phosphoribosyltransferase (QPRTase) is a major enzyme in the catabolism of quinolinate. Quinolinate is an intermediate in the tryptophan-nicotinamide adenine dinucleotide (NAD) pathway, leading to the production of nicotinic acid, carbon dioxide, and pyrophosphate. Catabolism of quinolinate is vital due to the quinolinate’s neurotoxicity. Increased levels of quinolinate have been linked to neurodegenerative symptoms associated with meningitis and AIDS. QAPRTase has a seven-stranded alpha/beta-barrel domain which is similar in structure to the eight-stranded alpha/beta-barrel enzymes. The protein possesses a novel fold in comparison to other members of the PRTase family. This fold comprises a structure combining two domains. The structure is part alpha/beta barrel-like domain, and part alpha/beta N-terminal domain.

Function:
Involved in the catabolism of quinolinic acid (QA).

Subunit:
Hexamer formed by 3 homodimers.

Similarity:
Belongs to the nadC/modD family.

SWISS:
Q15274

Gene ID:
23475

Database links:

Entrez Gene: 614254 Cow

Entrez Gene: 607664 Dog

Entrez Gene: 100727900 Guinea pig

Entrez Gene: 100065768 Horse

Entrez Gene: 23475 Human

Entrez Gene: 100406642 Marmoset (common)

Omim: 606248 Human

SwissProt: Q3T063 Cow

SwissProt: Q15274 Human

Unigene: 513484 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Quinolinate phosphoribosyltransferase; QPRT/QAPRTase) Polyclonal Antibody, Unconjugated (SL20316R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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