Rabbit Anti-TTI1 antibody | Sunlong Medical

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SMG10; Tel two interacting protein 1; Tel2 interacting protein 1 homolog (S. pombe); TEL2 interacting protein 1 homolog; TTI1_HUMAN.

Cat:

SL20332R

Species Reactivity：

Rat,(predicted: Human,Mouse,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human TTI1:81-180/1089

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T Cell Lysate at 40 ugMcf-7 Cell Lysate at 40 ugPrimary: Anti-TTI1 (SL20332R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 122 kDObserved band size: 122 kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TTI1) Polyclonal Antibody, Unconjugated (SL20332R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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TTI1 promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals. TTI1 interacts with TELO2, MTOR, ATM, ATR, PRKDC, SMG1 and TRRAP.

Function:
Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals.

Subunit:
Component of the TTT complex composed of TELO2, TTI1 and TTI2. Interacts with ATM, ATR, MTOR, PRKDC, SMG1, TELO2, TRRAP AND TTI2.

Tissue Specificity:
Widely expressed.

Similarity:
Belongs to the tti1 family.

SWISS:
O43156

Gene ID:
9675

Database links:

Entrez Gene: 534917 Cow

Entrez Gene: 9675 Human

Entrez Gene: 75425 Mouse

Entrez Gene: 100154931 Pig

Entrez Gene: 499935 Rat

Omim: 614425 Human

SwissProt: O43156 Human

SwissProt: Q91V83 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TTI1) Polyclonal Antibody, Unconjugated (SL20332R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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