Paraformaldehyde-fixed, paraffin embedded (Human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (STAT3) Polyclonal Antibody, Unconjugated (SL20382R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Sample:RSC96 cells(pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C)
Primary Antibody:Rabbit Anti-STAT3 antibody(SL20382R) (green);Concentration:0.2μg/10^6 cells
Isotype Control Antibody: Rabbit IgG (orange)
Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE (white blue)