Blank control (blue line): U251 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% Tween for 20 min at room temperature.).
Primary Antibody (green line): Rabbit Anti-ZO-1 antibody (SL23086R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG(SL0295P) .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC(SL0295G-FITC),Dilution: 1μg /test.
Blank control (blue line): U251 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-ZO-1 antibody (SL23086R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.