Rabbit Anti-LDL receptor antibody

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Low-density lipoprotein receptor precursor; LDL-R; LDL R; FH; FHC; LDL R; LDLR; Low Density Lipoprotein Receptor; Low density lipoprotein receptor familial hypercholesterolemia.

Cat:

SL20714R

Species Reactivity：

Human,Rat,(predicted: Mouse,Pig,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human LDL receptor:501-600/860<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LDL receptor) Polyclonal Antibody, Unconjugated (SL20714R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (SL20714R) ;Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.

Function:
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HISLV1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

Subunit:
Interacts with LDLRAP1. Interacts with SNX17. Interacts with HCV E1/E2 heterodimer. Interacts with HISLV1 Tat.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Membrane, clathrin-coated pit; Single-pass type I membrane protein. Note=Found distributed from the plasma membrane to intracellular compartments.

Tissue Specificity:
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HISLV1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

Post-translational modifications:
N- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation.

Similarity:
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats.

SWISS:
P01130

Gene ID:
3949

Database links:

Entrez Gene: 3949 Human

Entrez Gene: 16835 Mouse

Entrez Gene: 300438 Rat

Omim: 606945 Human

SwissProt: P01130 Human

SwissProt: P35951 Mouse

SwissProt: P35952 Rat

Unigene: 213289 Human

Unigene: 728190 Human

Unigene: 3213 Mouse

Unigene: 10483 Rat

低密度脂蛋白受体 LDLR是一种存在于细胞表面的、可识别多种配体的脂蛋白受体，在体内对于富含甘油三酯的脂蛋白代谢非常重要;
LDL R目前主要用于代谢及肿瘤方面的研究。 Picture

Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (SL20714R) ; Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.

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