A disintegrin and metalloproteinase domain 17; ADAM 17; ADAM metallopeptidase domain 17; ADAM17 protein; CD 156b; CD156b; CD156b antigen; CSVP; MGC71942; Snake venom like protease; TACE; TNF alpha convertase; TNF alpha converting enzyme; Tumor Necrosis Fa
Cat:
SLM52009R
Species Reactivity：
Human,Mouse,(predicted: Rat,)
Immunogen：
Recombinant human ADAM17 protein:700-824/824<Cytoplasmic>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:20-200IHC-F=1:20-200IF=1:20-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Pancreas (Mouse) Lysate at 40 ugPrimary: Anti-ADAM17 (9E7) (SLM52009R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 93 kDObserved band size: 93 kDSample: Spleen (Mouse) Lysate at 40 ugJurkat(Human) Cell Lysate at 30 ugSW96(Human) Cell Lysate at 30 ugPrimary: Anti- ADAM17 (9E7) (SLM52009R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 93 kDObserved band size: 100 kD
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq].
Subunit: Interacts with MAD2L1, MAPK14 and MUC1.
Subcellular Location: Membrane.
Tissue Specificity: Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-ADAM17 (9E7) (SLM52009R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 93 kD
Observed band size: 93 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Jurkat(Human) Cell Lysate at 30 ug
SW96(Human) Cell Lysate at 30 ug
Primary: Anti- ADAM17 (9E7) (SLM52009R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 93 kD
Observed band size: 100 kD
