Rabbit Anti-MAP4 antibody | Sunlong Medical

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DKFZp779A1753; MAP-4; MAP4; MAP4_HUMAN; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730.

Cat:

SL21935R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human MAP4:891-990/1156

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: A431(Human) Cell Lysate at 30 ugPrimary: Anti-MAP4 (SL21935R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 121 kDObserved band size: 121 kDParaformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP4) Polyclonal Antibody, Unconjugated (SL21935R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The MAP4 (Microtubule associated protein 4)is a non neuronal microtubule associated protein. It promotes microtubule assembly.The phosphorylation of the Pro rich region in the SLCterminus negatively regulates MAP4 activity to promote microtubule assembly.

Function:
Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

Subunit:
Interacts with SEPT2; this interaction impedes tubulin-binding.

Subcellular Location:
Cytoplasm, cytoskeleton.

Post-translational modifications:
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.

Similarity:
Contains 4 Tau/MAP repeats.

SWISS:
P27816

Gene ID:
4134

Database links:
Entrez Gene: 4134 Human

Entrez Gene: 17758 Mouse

Entrez Gene: 367171 Rat

Omim: 157132 Human

SwissProt: P27816 Human

SwissProt: P27546 Mouse

SwissProt: Q5M7W5 Rat

Unigene: 517949 Human

Unigene: 217318 Mouse

Unigene: 443428 Mouse

Unigene: 484451 Mouse

Unigene: 203122 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP4) Polyclonal Antibody, Unconjugated (SL21935R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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