TOPK; Cancer/testis antigen 84; CT84; FLJ14385; Lymphokine activated killer T cell originated protein kinase; MAPKK like protein kinase; PDZ-binding-kinase/T-LAK cell-originated protein kinase； Nori 3; Nori3; PDZ binding kinase; Serine/threonine protein k
KLH conjugated synthetic peptide derived from human PBK/TOPK:71-170/322
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Bone (Mouse) Lysate at 40 ugPrimary: Anti-PBK’TOPK (SL21872R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDSample: Bone (Mouse) Lysate at 40 ugPrimary: Anti- PBK/TOPK Dye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kD
This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Function: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Subunit : Interacts with DLG1 and TP53.
Tissue Specificity: Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Post-translational modifications: Phosphorylated; in a cell-cycle dependent manner at mitosis.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-PBK’TOPK (SL21872R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti- PBK/TOPK Dye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
