Rabbit Anti-AATF antibody | Sunlong Medical

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Apoptosis antagonizing transcription factor; CHE1; DED; AATF_HUMAN; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1; Protein AATF;Rb binding protein Che 1.

Cat:

SL1229R

Species Reactivity：

Human,(predicted: Mouse,Rat,Pig,Cow,Horse,Rabbit,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human AATF:451-560/560

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-AATF Polyclonal Antibody, Unconjugated(SL1229R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008]

Function:
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.

Subunit:
Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT.

Subcellular Location:
Nucleus, nucleolus.

Tissue Specificity:
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.

Post-translational modifications:
Hyperphosphorylated during the G1/S phase transition.

Similarity:
Belongs to the AATF family.

SWISS:
Q9NY61

Gene ID:
26574

Database links:

Entrez Gene: 26574 Human

Entrez Gene: 56321 Mouse

Omim: 608463 Human

SwissProt: Q9NY61 Human

SwissProt: Q3URN5 Mouse

SwissProt: Q9JKX4 Mouse

Unigene: 195740 Human

Unigene: 257482 Mouse

AATF属于细胞凋亡的拮抗转录因子,有抑制细胞凋亡的发展过程。 Picture

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-AATF Polyclonal Antibody, Unconjugated(SL1229R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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