Rabbit Anti-ADAMTS1 antibody

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A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin like and metalloprotease with thrombospondin type 1 motif 1; A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1; ADAM metallopeptidase

Cat:

SL1208R

Species Reactivity：

Human,(predicted: Mouse,Rat,Chicken,Pig,Cow,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse ADAMTS1:851-967/967

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Human Hela cell Lysates Lane 2: Human 293T cell Lysates Lane 3: Human U87-MG cell Lysates Lane 4: Human U251 cell Lysates Primary: Anti-ADAMTS1 (SL1208R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 106kDaObserved band size: 80kDaTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(SL1208R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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ADAMTS1 is a metalloproteinase of the ADAM (A Disintegrin And Metalloproteinase) family containing disintegrin-like domains. ADAMTS1, also known as METH1, was first described as a protein elevated in invasive mouse tumors. Initial findings indicated a role for ADAMTS1 in tumor progression, since the protein was preferentially expressed in more invasive tumor cell lines. ADAMTS1 is necessary for normal growth, fertility, and organ morphology and function.

Function:
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.

Subcellular Location:
Secreted > extracellular space > extracellular matrix.

Post-translational modifications:
The precursor is cleaved by a furin endopeptidase.

Similarity:
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains.

SWISS:
P97857

Gene ID:
11504

Database links:

Entrez Gene: 9510 Human

Entrez Gene: 11504 Mouse

Entrez Gene: 79252 Rat

Omim: 605174 Human

SwissProt: Q9UHI8 Human

SwissProt: P97857 Mouse

SwissProt: Q9WUQ1 Rat

Unigene: 643357 Human

Unigene: 8230 Human

Unigene: 1421 Mouse

Unigene: 7897 Rat

Picture

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(SL1208R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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