Rabbit Anti-Bassoon antibody

Home > Product > Antibody > Rabbit Anti-Bassoon antibody

Bassoon; BSN; KIAA0434; Neuronal double zinc finger protein; Presynaptic cytomatrix protein; Protein bassoon; Zinc finger protein 231; ZNF 231; ZNF231; Neuronal double zinc finger protein; Zinc-finger protein 231; BSN_HUMAN; ZNF 231.

Cat:

SL0275R

Species Reactivity：

Mouse,(predicted: Human,Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human BSN:2901-3000/3926

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Bassoon Polyclonal Antibody, Unconjugated(SL0275R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses. [provided by RefSeq, Jul 2008].

Function:
Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses.

Subunit:
Interacts with ERC2/CAST1, RIMS1 and UNC13A. Part of a complex consisting of ERC2, RIMS1 and BSN.

Subcellular Location:
Cytoplasm. Cell junction, synapse, synaptosome. Cytoplasm, cytoskeleton. Note=Localized to the active zone of presynaptic density.

Tissue Specificity:
Exclusively expressed in brain.

Post-translational modifications:
Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization.

SWISS:
Q9UPA5

Gene ID:
8927

Database links:

Entrez Gene: 8927 Human

Entrez Gene: 12217 Mouse

Entrez Gene: 29138 Rat

Omim: 604020 Human

SwissProt: Q9UPA5 Human

SwissProt: O88737 Mouse

SwissProt: O88778 Rat

Unigene: 194684 Human

Unigene: 20425 Mouse

Unigene: 29999 Rat

锌指蛋白是80年代中期发现的一类DNA结合蛋白，在真核生物中,锌指蛋白可能是最大的一类DNA结合蛋白，并且由锌指蛋白调控基因表达是发育和其他过程的一个非常普遍的现象。在最近的几年中，发现某些锌指蛋白对肌肉的发育具有重要的调节作用。 Picture

Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Bassoon Polyclonal Antibody, Unconjugated(SL0275R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Product Feedback Wall