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Home > Product > Antibody > Rabbit Anti-KCNMA1/BK channel antibody
Maxi Potassium channel alpha; bA205K10.1; BK channel; BKCa channels; BKCA alpha; BKCA alpha subunit; BKTM; Calcium activated potassium channel subfamily M subunit alpha 1; Calcium activated potassium channel subunit alpha 1; DKFZp686K1437; Drosophila slow
Cat:
SL0868R
Species Reactivity：
Rat,(predicted: Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep,)
Immunogen：
KLH conjugated synthetic peptide derived from human BK channel:1151-1236/1236<Cytoplasmic>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
IHC-P=1:100-500IHC-F=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KCNMA1/BK channel) Polyclonal Antibody, Unconjugated (SL0868R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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Product PDFs
Datasheet:


MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].

Function:
Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).

Subunit:
Homotetramer.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Widely expressed. Except in myocytes, it is almost ubiquitously expressed.

Post-translational modifications:
Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.

DISEASE:
Defects in KCNMA1 are the cause of generalized epilepsy and paroxysmal dyskinesia (GEPD) [MIM:609446]. Epilepsy is one of the most common and debilitating neurological disorders. Paroxysmal dyskinesias are neurological disorders characterized by sudden, unpredictable, disabling attacks of involuntary movement often requiring life-long treatment. The coexistence of epilepsy and paroxysmal dyskinesia in the same individual or family is an increasingly recognized phenomenon. Patients manifest absence seizures, generalized tonic-clonic seizures, paroxysmal nonkinesigenic dyskinesia, involuntary dystonic or choreiform movements. Onset is usually in childhood and patients may have seizures only, dyskinesia only, or both.

Similarity:
Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.
Contains 1 RCK N-terminal domain.

SWISS:
Q12791

Gene ID:
3778

Database links:

Entrez Gene: 3778 Human

Entrez Gene: 16531 Mouse

Entrez Gene: 83731 Rat

Omim: 600150 Human

SwissProt: Q12791 Human

SwissProt: Q0892 Mouse

SwissProt: Q62976 Rat

Unigene: 144795 Human

Unigene: 34727 Mouse

Unigene: 486347 Mouse

Unigene: 30616 Rat



通道蛋白（Channel Protein） Picture

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KCNMA1/BK channel) Polyclonal Antibody, Unconjugated (SL0868R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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