NAIF protein 1 (nuclear apoptosis inducing factor 1; CB12-327; RP-379C10.2) belongs to the NAIF1 family. Apoptosis is a genetically determined cell suicidal pro gram that plays critical roles in many physiological and pathological processes. nuclear apoptosis-inducing factor 1 (NAIF1), overexpression of which induces apoptosis in cells. Human NAIF1 is located on chromosome 9q34.11 and encodes 327 amino acids with a homeodomain-like region and two nuclear localization signals at its N-terminal region. NAIF1 is conserved across diverse species, including human, mouse, crab-eating macaque, dog, chicken and frog, and shares no obvious homology to any known genes or proteins. Northern blot analysis revealed wide expression of NAIF1 mRNA throughout human tissues. NAIF1 was predominantly localized in the nucleus. Overexpression of NAIF1 inhibited cell growth and induced apoptosis. Furthermore, NAIF1 transfection caused both decreases in mitochondrial membrane potential and caspase-3 activation. In summary, NAIF1 is a nuclear protein that induces apoptosis when overexpressed.
Function:
Induces apoptosis.
Subunit:
Interacts with HARBI1.
Subcellular Location:
Nucleus.
Tissue Specificity:
Widely expressed.
Similarity:
Belongs to the NAIF1 family.
SWISS:
Q69YI7
Gene ID:
203245
Database links:
Entrez Gene: 510689 Cow
Entrez Gene: 203245 Human
Entrez Gene: 71254 Mouse
Omim: 610673 Human
SwissProt: A7MSH3 Cow
SwissProt: Q9BE21 Cynomolgus Monkey
SwissProt: Q69YI7 Human
SwissProt: Q6PFD7 Mouse
Unigene: 37726 Human
Unigene: 102341 Mouse
NAIF1 protein(CB12-327)是最新发现的大肠癌标志物他抑制NFκB，NFAT，AP-1活性，并诱导细胞调亡。
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Tissue/cell: human colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-NAIF1 Polyclonal Antibody, Unconjugated(SL0452R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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