Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Heart (Rat) Lysate at 40 ug
Lane 3: Large intestine (Rat) Lysate at 40 ug
Lane 4: Stomach (Rat) Lysate at 40 ug
Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 6: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-TrkA(Tyr674 + Tyr675) + TrkB(Tyr706 + Tyr707) (SL3457R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140/110 kD
Observed band size: 120 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-TrkA(Tyr674/675)/TrkB(Tyr706/707) Polyclonal Antibody, Unconjugated (SL3457R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining