Rabbit Anti-GDNFRA antibody

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GDNF Receptor alpha 1; GFRa-1; GFR alpha; GDNFR alpha; TmR1; GFR alpha-1; RETL1; RET ligand 1; TGF-beta related neurotrophic factor receptor-1; AU042498; DKFZp313E1012; DKFZp686J0156; FLJ10561; FLJ31546; GDNFR; Gdnfr alpha; GFR-ALPHA-1; GFRA1; GRFA1; LOC1

Cat:

SL0201R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human GFR Alpha-1:351-429/465

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: Human colon cancer ; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GDNFRA Polyclonal Antibody, Unconjugated(SL0201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]

Function:
Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.

Subunit:
2 molecules of GDNFR-alpha are thought to form a complex with the disulfide-linked GDNF dimer and with 2 molecules of RET.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Similarity:
Belongs to the GDNFR family.

SWISS:
P56159

Gene ID:
2674

Database links:

Entrez Gene: 2674 Human

Entrez Gene: 14585 Mouse

Entrez Gene: 25454 Rat

Omim: 601496 Human

SwissProt: P56159 Human

SwissProt: P97785 Mouse

SwissProt: Q62997 Rat

Unigene: 591913 Human

Unigene: 88489 Rat

GFRα-1是由68个氨基酸组成的蛋白，广泛表达于中枢神经系统和外周器官。活性亚单位由两个GFRα-1分子中间由二硫键连接而成的复合物。属于GDNFR 家族成员。 Picture

Tissue/cell: Human colon cancer ; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GDNFRA Polyclonal Antibody, Unconjugated(SL0201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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