Rabbit Anti-CCL3 antibody | Sunlong Medical

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Tonsillar lymphocyte LD78 alpha protein; CCL 3; CCL3; Chemokine C C motif ligand 3; Chemokine CC Motif Ligand 3; G0/G1 switch regulatory protein 19 1; G0S19 1; G0S19 1 protein; Heparin binding chemotaxis protein antibody L2G25B; LD78ALPHA; Macrophage inf

Cat:

SL1045R

Species Reactivity：

Rat,(predicted: Human,Mouse,)

Immunogen：

KLH conjugated synthetic peptide derived from human MIP-1 alpha:41-92/92

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: rat exfoliated cells; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MIP-1 Alpha/CCL3 Polyclonal Antibody, Unconjugated(SL1045R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the SLCC chemokine receptor-1 (SLCC CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.

Function:
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HISLVsuppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HISLV1, HISLV2, and simian immunodeficiency virus (SIV).

Subunit:
Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69).

Subcellular Location:
Secreted.

Post-translational modifications:
N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.

Similarity:
Belongs to the intercrine beta (chemokine CC) family.

SWISS:
P10147

Gene ID:
6348

Database links:

Entrez Gene: 6348 Human

Entrez Gene: 20302 Mouse

Entrez Gene: 25542 Rat

Omim: 182283 Human

SwissProt: P10147 Human

SwissProt: P10855 Mouse

SwissProt: P50229 Rat

Unigene: 514107 Human

Unigene: 1282 Mouse

Unigene: 10139 Rat

巨噬细胞炎症蛋白1α（MIP-1α）是CC趋化因子中的一种。主要用于炎症、肿瘤方面的研究。 Picture

Tissue/cell: rat exfoliated cells; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MIP-1 Alpha/CCL3 Polyclonal Antibody, Unconjugated(SL1045R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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