Rabbit Anti-Alpha casein antibody

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Casein; alpha-S1-casein; CASA; CASA1_Cow; Casein alpha s1; casein, alpha; casein, alpha-S1, included; Casoxin-D; CSN1; CSN1S1; MGC149368.

Cat:

SL0813R

Species Reactivity：

(predicted: Mouse,Cow,)

Immunogen：

Casein from bovine milk

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti-Casein (SL0813R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 24-26 kD

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Casein is the name for a family of related phosphoproteins (αS1, αS2, β, κ). These proteins are commonly found in mammalian milk, making up 80% of the proteins in cow milk and between 20% and 45% of the proteins in human milk. Casein has a wide variety of uses, from being a major component of cheese, to use as a food additive, to a binder for safety matches. As a food source, casein supplies amino acids; carbohydrates; and two inorganic elements, calcium and phosphorus.

Subunit:
Casein contains a fairly high number of proline residues, which do not interact. There are also no disulfide bridges. As a result, it has relatively little tertiary structure. It is relatively hydrophobic, making it poorly soluble in water. It is found in milk as a suspension of particles called "casein micelles" which show only limited resemblance with surfactant-type micellae in a sense that the hydrophilic parts reside at the surface and they are spherical. However, in sharp contrast to surfactant micelles, the interior of a casein micelle is highly hydrated. The caseins in the micelles are held together by calcium ions and hydrophobic interactions. Several models account for the special conformation of casein in the micelles. One of them proposes the micellar nucleus is formed by several submicelles, the periphery consisting of microvellosities of κ-casein. Another model suggests the nucleus is formed by casein-interlinked fibrils. Finally, the most recent model proposes a double link among the caseins for gelling to take place. All three models consider micelles as colloidal particles formed by casein aggregates wrapped up in soluble κ-casein molecules.
The isoelectric point of casein is 4.6. Since milk's pH is 6.6, casein has a negative charge in milk. The purified protein is water insoluble. While it is also insoluble in neutral salt solutions, it is readily dispersible in dilute alkalis and in salt solutions such as sodium oxalate and sodium acetate.
The enzyme trypsin can hydrolyze off a phosphate-containing peptone. It is used to form a type of organic adhesive.

SWISS:
N/A

Gene ID:
282208

Database links:
Entrez Gene: 282208 Bovin

Entrez Gene: 1446 Human

Omim: 115450 Human

SwissProt: P47710 Human

Unigene: 3155 Human

酪蛋白(casein)是牛乳包括牛初乳的主要成分，通常在分离提取乳清过程中可得到大量的酪蛋白，它具有各种必需氨基酸，且组分合理，是一种优质的动物蛋白。然而，由于人体尤其是儿童对牛乳酪蛋白的分解能力较弱，牛乳酪蛋白的应用并不广泛。近来国外文献报道，牛乳酪蛋白短链肽除了易吸收外，还具有某些重要的生物学功能，提示着酪蛋白除单纯营养作用之外的广阔应用前景。 Picture

Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-Casein (SL0813R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 24-26 kD

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Specific References (19) | SL0813R has been referenced in 19 publications.

[IF=5.396] Riguo Lan. et al. Reduction of ROS-HIF1α-driven glycolysis by taurine alleviates Streptococcus uberis infection. Food Funct. 2022 Jan;: WB ; Mouse.

PubMed:35112684

[IF=3.612] Lulu Wang. et al. METTL3 is a key regulator of milk synthesis in mammary epithelial cells. 2021 Dec 11 WB ; Bovine ,Mouse.

PubMed:34865263

[IF=25.841] Chaoxiang Chen. et al. Active cargo loading into extracellular vesicles: Highlights the heterogeneous encapsulation behaviour. J Extracell Vesicles. 2021 Nov;10(13):e12163 FC ; Material.

PubMed:34719860

[IF=2.985] Xiaohan Yuanet al. NCOA5 is a master regulator of amino acid-induced mTOR activation and β-casein synthesis in bovine mammary epithelial cells. Biochem Biophys Res Commun . 2020 Aug 27;529(3):569-574. WB ; cow.

PubMed:32736675

[IF=3.263] Yang Yu et al. Vaccarin promotes proliferation of and milk synthesis in bovine mammary epithelial cells through the Prl receptor-PI3K signaling pathway. Eur J Pharmacol. 2020 Aug 5;176:173190. WB ; dairy cow.

PubMed:32464193

[IF=2.705] Luo C et al. Methionine stimulates GlyRS phosphorylation via the GPR87-CDC42/Rac1-MAP3K10 signaling pathway. Biochem Biophys Res Commun. 2020 Mar 19;523(4):847-852. ICF ; dairy cow.

PubMed:31954518

[IF=3.571] Dai W et al. ASCT2 is involved in SARS-mediated β-casein synthesis of bovine mammary epithelial cells with methionine supply. J Agric Food Chem. 2019 Oct 22. WB ; Cow.

PubMed:31597423

[IF=3.89] Zhang, Minghui, et al. "Annexin A2 Positively Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells through the mTOR Signaling Pathway." Journal of Cellular Physiology (2017). WB ; Human.

PubMed:28771726

[IF=0.89] Zhen, Zhen, et al. "DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells." In Vitro Cellular & Developmental Biology-Animal (2017): 1-9. WB ; Bovine.

PubMed:28842848

[IF=3.54] Zhang, Boce, Ariella Feldman, and Qin Wang. "A novel insight in rapid allergen detection in food systems: From threshold dose to real-world concentration."Sensors and Actuators B: Chemical?186 (2013): 597-602. ELISA ;

PubMed:doi:10.1016/j.snb.2013.07.055

[IF=1.857] Lampasona Timothy. et al. Behavioral Effects and Retention of Protein Immunomarkers on Plum Curculio Conotrachelus nenuphar (Coleoptera: Curculionidae). J Insect Sci. 2021 Nov;21(6): ELISA ; Insects.

PubMed:34741456

[IF=1.487] Mengmeng Yu. et al. Daidzein promotes milk synthesis and proliferation of mammary epithelial cells via the estrogen receptor α-dependent NFκB1 activation. 2020 May 13 WB ; Bovine.

PubMed:3481613

[IF=6.895] Chaoxiang Chen. et al. General and mild modification of food-derived extracellular vesicles for enhanced cell targeting. Nanoscale. 2021 Feb;13(5):3061-3069

PubMed:3352366

[IF=3.571] Yu M et al. Taurine promotes milk synthesis via the GPR87-PI3K-SETD1A signaling in BMECs. J Agric Food Chem. 2019 Feb 20;67(7):1927-1936. WB ; Mouse.

PubMed:30678459

[IF=3.923] Huo N et al. PURB is a positive regulator of amino acid‐induced milk synthesis in bovine mammary epithelial cells.(2018) J Cell Physiol. Oct 26. WB ; dairy cow.

PubMed:30362557

[IF=3.412] Qi H et al. Methionine Promotes Milk Protein and Fat Synthesis and Cell Proliferation via the SNAT2-PI3K Signaling Pathway in Bovine Mammary Epithelial Cells.(2018) J Agric Food Chem.66(42):11027-11033. IF,WB ; dairy cow.

PubMed:30274521

[IF=3.923] Zhang S et al. The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs. (2018) J. Cell. Physiol. Sep 06 WB ; human.

PubMed:30187485

[IF=1.44] Blaauw, Brett R., et al. "Measuring host plant selection and retention of Halyomorpha halys by a trap crop." Entomologia Experimentalis et Applicata.

PubMed:10.1111/eea.12571

[IF=2.1] Blaauw, Brett R., Vincent P. Jones, and Anne L. Nielsen. "Utilizing immunomarking techniques to track Halyomorpha halys (Hemiptera: Pentatomidae) movement and distribution within a peach orchard." PeerJ 4 (2016): e1997. ELISA ; Others.

PubMed:27190711