Rabbit Anti-Granzyme B antibody

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GranzymeB; Granzyme-B; C11; Cathepsin G-like 1; CTLA-1; Cathespin G Like 1; CCPI; CCP-1; CGL 1; CGL1; CSP B; CSPB; CTLA 1;CTLA1; CTSGL 1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T Lymphocyte Associated Serine Esterase 1; Cytotoxic T lymphocyte pro

Cat:

SL1351R

Species Reactivity：

Human,(predicted: Mouse,Rat,Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human GZMB:21-130/247

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=2ug/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Granzyme B Polyclonal Antibody, Unconjugated(SL1351R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control:U937. Primary Antibody (green line): Rabbit Anti-Granzyme B antibody (SL1351R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

Function:
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Subcellular Location:
Cytoplasmic granule. Note=Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

Similarity:
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.

SWISS:
P10144

Gene ID:
3002

Database links:

Entrez Gene: 3002 Human

Entrez Gene: 14939 Mouse

Entrez Gene: 171528 Rat

Omim: 123910 Human

SwissProt: P10144 Human

SwissProt: P04187 Mouse

SwissProt: P18291 Rat

Unigene: 1051 Human

Unigene: 14874 Mouse

Unigene: 132115 Rat

Unigene: 145789 Rat

Unigene: 21395 Rat

Granzyme B—是一种丝氨酸蛋白酶,作用在细胞凋亡中。 Picture

Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Granzyme B antibody (SL1351R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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