Caspases are cysteine proteases that cleave SLCterminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011]
Function:
Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.
Tissue Specificity:
Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.
Similarity:
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
SWISS:
Q6UXS9
Gene ID:
100506742
Database links:
Entrez Gene: 100506742 Human
SwissProt: Q6UXS9 Human
Unigene: 476989 Human
ASPASE-12是caspase家族成员，是存在于内质网上的促凋亡因子，内质网应激早期可通过GRP（glucose-regulated protein，GRP）的表达增多来保护细胞；CASPASE-12是内质网细胞关键介导因子，caspase-12还是caspase-1的抑制剂。
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-12 Polyclonal Antibody, Unconjugated(SL0967R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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