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Home > Product > Antibody > Rabbit Anti-RBBP6 antibody
Retinoblastoma-binding protein 6; proliferation potential protein related; p53-associated cellular protein of testis; Proliferation potential-related protein; Retinoblastoma-binding Q protein 1; Protein RBQ-1; My038; PACT; Protein P2P-R; RBBP6_HUMAN; P2P-
Cat:
SL1483R
Species Reactivity：
Mouse,(predicted: Human,Rat,)
Immunogen：
KLH conjugated synthetic peptide derived from human RBBP6:2-150/1792
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBBP6) Polyclonal Antibody, Unconjugated (SL1483R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product PDFs
Datasheet:


he retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth.

Subunit:
Interacts with p53/TP53 and RB1. Interacts also with MDM2 and YBX1. Interacts with NEK6.

Subcellular Location:
Nucleus, nucleolus. Chromosome. Cytoplasm, cytoskeleton, centrosome. Note=Colocalizes with mitotic chromosomes. Co-localizes with NEK6 in the centrosome.

Tissue Specificity:
Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.

Post-translational modifications:
Phosphorylated by NEK6.

Similarity:
Contains 1 CCHSLCtype zinc finger.
Contains 1 DWNN domain.
Contains 1 RING-type zinc finger.

SWISS:
Q7Z6E9

Gene ID:
5930

Database links:

Entrez Gene: 5930 Human

Entrez Gene: 19647 Mouse

Entrez Gene: 308968 Rat

Omim: 600938 Human

SwissProt: Q7Z6E9 Human

SwissProt: P97868 Mouse

Unigene: 188553 Human

Unigene: 496 Mouse



信号传导（Signaling Intermediates）
P2P-P又称PACT即P53相关细胞蛋白（P53 associated cellular protein-testes derived）， P2P-P蛋白是p53的负调控因子，而且在细胞生长和胚胎发育，细胞分化、前体mRNA剪接、周期阻滞、凋亡过程中起到了重要作用。 Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBBP6) Polyclonal Antibody, Unconjugated (SL1483R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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