[email protected]
Home > Product > Antibody > Rabbit Anti-BIRC6 antibody
BIRC6; Apollon; Apollon protein; Baculoviral IAP repeat containing 6; Baculoviral IAP repeat containing protein 6; BIRC 6; BRUCE; FLJ13726; FLJ13786; KIAA1289; Ubiquitin conjugating BIR domain enzyme apollon; BIRC6_HUMAN.
Cat:
SL1489R
Species Reactivity：
Human,(predicted: Mouse,Rat,Pig,Cow,Horse,Rabbit,)
Immunogen：
KLH conjugated synthetic peptide derived from human Apollon C terminus:4701-4857/4857
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BIRC6) Polyclonal Antibody, Unconjugated (SL1489R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
More
prev next
Unit:
Price: $
Product PDFs
Datasheet:


Conserved genes that encode inhibitors of apoptosis proteins (IAPs) are frequently overexpressed in human cancers and are associated with resistance to therapy. IAPs contain a 70 amino acid cysteine and histidine rich baculovirus IAP repeat (BIR) and often a zinc binding RING finger. Chen et al. (1999) found that some brain cancer cell lines with high expression of BIRC6 exhibited resistance to apoptosis in the presence of anticancer drugs. Treatment of a high expressing cell line with BIRC6 antisense oligonucleotides reduced BIRC6 expression and reversed the resistance to anticancer drugs.

Function:
Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification.

Subunit:
Homodimer. Binds the activated, processed forms of CASP3, CASP6, CASP7 and CASP9. Interacts with RNF41, DIABLO/SMAC, HTRA2, KIF23/MKLP1, USP8/UBPY, BIRC5/survivin, MAP2K1/MEK1, RAB8A/RAB8, RAB11A/RAB11, PLK1, EXOC3/SEC6 and EXOC4/SEC8.

Subcellular Location:
Golgi apparatus, trans-Golgi network membrane. Midbody. Endosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, centrosome. Note=Concentrates in a pericentriolar compartment in interphase, moves partially to spindle poles in metaphase, and finally localizes to the spindle midzone and the midbody in telophase and during cytokinesis.

Tissue Specificity:
Expressed in brain cancer cells.

Post-translational modifications:
Ubiquitinated. Ubiquitination is mediated by the RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis. Deubiquitinated by USP8/UBPY.

Similarity:
In the SLCterminal section; belongs to the ubiquitin-conjugating enzyme family.
Contains 1 BIR repeat.

SWISS:
Q9NR09

Gene ID:
57448

Database links:

Entrez Gene: 57448 Human

Entrez Gene: 12211 Mouse

Omim: 605638 Human

SwissProt: Q9NR09 Human

SwissProt: O88738 Mouse

Unigene: 150107 Human

Unigene: 290908 Mouse



Apollon是凋亡抑制蛋白家族IAP(inhibitors of apoptosis protein 凋亡抑制因子)的成员之一，它通过抑制Smac和caspase-9酶活性而产生抗凋亡作用它是IAP家族中分子量最大的成员,Apollon在某些肿瘤组织中表达。 Picture

Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BIRC6) Polyclonal Antibody, Unconjugated (SL1489R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Product Feedback Wall
Message :
Your Email :
Copyright © 2007-2018 Sunlong Medical All Rights Reserved.