Rabbit Anti-Human Serum albumin (Loading Control) antibody

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ALB; ALBU_HUMAN; Albumin (32 AA); Albumin (AA 34); Analbuminemia; Bisalbuminemia; Cell growth inhibiting protein 42; DKFZp779N1935; Dysalbuminemic hyperthyroxinemia; Growth inhibiting protein 20; HSA; Hyperthyroxinemia dysalbuminemic; PRO0883; PRO0903; PR

Cat:

SL0945R

Species Reactivity：

Human,Mouse,Rat,Goat,(predicted: Dog,)

Immunogen：

human Serum albumin puritied

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Serum (Human) at 20 ugLane 2: Serum (Mouse) at 20 ugLane 3: Serum (Rat) at 20 ugLane 4: Serum (Goat) at 20 ugLane 5: Liver (Rat) tissue lysates at 40 ugPrimary: Anti-Human Serum albumin (SL0945R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 69 kDObserved band size: 65 kDSample:HepG2 Cell (Human) Lysate at 40 ugPrimary: Anti-Serum albumin (SL0945R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 69 kDObserved band size: 69 kDHepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Albumin) polyclonal Antibody, Unconjugated (SL0945R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Unit:

Price: $136.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008].

Function:
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

Subcellular Location:
Secreted.

Tissue Specificity:
Plasma.

Post-translational modifications:
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid.

DISEASE:
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:10720]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.

Similarity:
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.

SWISS:
P02769

Gene ID:
280717

Database links:

Entrez Gene: 213 Human

Omim: 10720 Human

SwissProt: P02768 Human

Unigene: 418167 Human

Picture

Sample:HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-Serum albumin (SL0945R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Albumin) polyclonal Antibody, Unconjugated (SL0945R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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